The coconut rhinoceros beetle (CRB) is a large scarab beetle that is native to Southeast Asia. Key words: Coconut rhinoceros beetle diagnostic assay multiplex PCR oriental flower beetle. Coconut Rhinoceros Beetle ( Oryctes rhinoceros) Family: Scarabaeidae. This assay also features an internal positive control to ensure DNA of sufficient quantity and quality is used in the assay, increasing its reliability and reducing the chances of false negative results. Here, we report the development of a multiplex PCR assay targeting cytochrome oxidase I of coconut rhinoceros beetle and oriental flower beetle that can rapidly detect and distinguish between these insects. Hawaii’s invasive species experts announced in January that Oahu had lost its fight to eradicate the coconut rhinoceros beetle, after years of trying to keep their expansion at bay. Anyone who finds coconut rhinoceros beetles should contact the CRB Response Team at 80 or email, or the state’s toll-free Pest Hotline at 808-643-PEST (7378). Eggs, early life stages, and fecal excrement of coconut rhinoceros beetle and oriental flower beetle are morphologically indistinguishable, thereby creating uncertainty when such specimens are discovered in the field. Kauai County has closed the Lihue green waste transfer station, where the two beetles were found, in order to allow for the area to be surveyed. This pest was first detected in Honolulu in December, 2013, and aresponse was launched. Confounding this program is the widespread presence of another scarab beetle on Oahu, the oriental flower beetle, Protaetia orientalis (Gory and Percheron 1833). The coconut rhinoceros beetle, Oryctes rhinoceros, is a large scarab beetle which is native to Southeast Asia and is distributed through the Western Pacific Region. An incipient coconut rhinoceros beetle population was recently discovered on the island of Oahu, Hawaii and is currently the target of a large, mutiagency eradication program. I’ve heard it estimated that there are 100,000 coconut rhinoceros beetles on Oahu. Homeless in Hawaii 911 Report Volcanic Ash. The coconut rhinoceros beetle, Oryctes rhinoceros (L.), is a major pest of coconut and other palm trees. Traffic Map Weather Video Photo Galleries Hawaii News. (David Croxford/Civil Beat/2023) This is the first confirmed detection on Maui, though no live beetles have been found.
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